DIAPRO HAV AB ELISA KIT

DIAPRO HAV AB ELISA KIT Specification

Function
Detection of anti-HAV antibodies

Usage Type
Laboratory/Diagnostic Use

Features
High specificity and sensitivity, complete kit with ready to use reagents, suitable for clinical and diagnostic use

Shelf Life
12 months

Instruments Type
IVD (In Vitro Diagnostic)

Accuracy
>99%

Storage Instructions
Store at 2-8C (Refrigerated)

Measurement Range
Qualitative results

Frequency
Single use

Equipment Type
ELISA Kit

Material
High-quality plastic reagents and microplates

Condition
New

Technology
Enzyme-Linked Immunosorbent Assay (ELISA)

Portable
Yes

Wall Mounted
No

Real-Time Operation
No

Noise Level
Silent Operation

Operating Type
Manual

Use
For qualitative detection of total antibodies against Hepatitis A Virus in human serum or plasma

Dimension (L*W*H)
Standard kit size

Weight
Approx. 1 kg

Color
White kit with labeled reagents

Intended User
Trained laboratory professionals

Kit Components
Microtiter plate, conjugate, substrate, control, wash buffer, instruction manual

Incubation Time
Approximately 60 minutes total

Compatible Plate Reader
Required (450 nm filter)

Specificity
>99%

Sensitivity
>99%

Sample Type
Human serum or plasma

Number of Tests per Kit
96 tests

About DIAPRO HAV AB ELISA KIT

Dia.Pro HAV Ab ELISA Kit

The DIAPRO HAV AB ELISA Kit is a competitive enzyme immunoassay (ELISA) designed to detect antibodies to Hepatitis A Virus (HAV) in human plasma and sera. The kit is used for the follow-up of patients infected by HAV.

Key Features:

Competitive ELISA format
Detects antibodies to HAV in human plasma and sera
Used for the follow-up of patients infected by HAV
In vitro diagnostic use only

Principle:

The assay is based on the principle of competition, where the antibodies in the sample compete with an anti-HAV conjugate for binding to a fixed amount of HAV antigen coated onto the microtiter plate wells. The amount of conjugate bound is inversely proportional to the amount of antibodies to HAV present in the sample.

Components:

Microtiter plate coated with HAV antigen
Anti-HAV conjugate
Sample diluent
Wash buffer
Substrate/chromogen mixture
Stop solution

Procedure:

Add sample diluent to the sample and mix well.
Add 100 L of the sample to the corresponding well of the microtiter plate.
Add 50 L of anti-HAV conjugate to each well.
Incubate for 1 hour at room temperature.
Wash the wells with wash buffer.
Add 100 L of substrate/chromogen mixture to each well.
Incubate for 30 minutes at room temperature.
Add 50 L of stop solution to each well.
Measure the optical density (OD) at 450 nm using an ELISA reader.

Interpretation:

The OD value is inversely proportional to the amount of antibodies to HAV present in the sample. A positive result indicates the presence of antibodies to HAV, while a negative result indicates the absence of detectable antibodies.

Note:

The DIAPRO HAV AB ELISA Kit is intended for in vitro diagnostic use only and should be used by trained laboratory personnel according to the manufacturers instructions.

Accurate Detection of HAV Antibodies

This ELISA kit provides sensitive and specific detection of anti-Hepatitis A Virus antibodies in human serum or plasma. With over 99% accuracy in both sensitivity and specificity, laboratories can trust the results for diagnostic and screening purposes. The complete kit includes all required reagents, reducing the risk of cross-contamination and ensuring consistency every test cycle.

User-Friendly and Ready-to-Use Kit

The DIAPRO HAV AB ELISA Kit is supplied with pre-labeled, ready-to-use components packaged in a compact, durable white kit. Detailed instructions allow trained laboratory professionals to easily prepare, execute, and interpret the assay manually. With minimal equipment required beyond a standard 450 nm plate reader, this kit enables efficient workflow integration for diagnostic settings.

FAQ's of DIAPRO HAV AB ELISA KIT:

Q: How is the DIAPRO HAV AB ELISA Kit used in a laboratory setting?

A: The kit is designed for trained laboratory professionals to qualitatively detect total antibodies against Hepatitis A Virus in human serum or plasma. After adding prepared samples and reagents to the microtiter plate, incubation and washing steps are performed as outlined in the manual, followed by reading results using a 450 nm plate reader.

Q: What sample types are compatible with this ELISA kit?

A: The DIAPRO HAV AB ELISA Kit is validated for use with human serum or plasma samples, making it suitable for most clinical and diagnostic applications.

Q: When should the kit be used for best results?

A: For optimal performance, the kit should be used within its 12-month shelf life and stored consistently at 2-8C. Consult the kit's packaging for the expiration date prior to use.

Q: Where can the DIAPRO HAV AB ELISA Kit be utilized?

A: This kit is ideal for use in clinical laboratories, diagnostic facilities, or research institutions equipped with standard ELISA equipment, such as a compatible 450 nm filter plate reader.

Q: What is the process for obtaining results with this kit?

A: Following sample and reagent addition, the assay requires about 60 minutes for incubation and processing. Results are then interpreted using a plate reader at 450 nm, providing qualitative detection of HAV antibodies.

Q: What are the key benefits of using the DIAPRO HAV AB ELISA Kit?

A: Key advantages include its high sensitivity and specificity (>99%), complete ready-to-use format, silent operation, portability, and suitability for various laboratory and diagnostic applications, ensuring reliable detection of HAV antibodies.

Q: How should the DIAPRO HAV AB ELISA Kit be stored and handled?

A: Keep the kit refrigerated at 2-8C and always consult the instruction manual for proper handling. After each use, reseal components tightly and avoid repeated freeze-thaw cycles to maintain reagent integrity.