DIACLONE HUMAN IL-6 ELISA KIT

DIACLONE HUMAN IL-6 ELISA KIT Specification

Shelf Life
12 months from date of manufacture

Features
High sensitivity, ready-to-use reagents, includes standard and controls, 96-well format

Measurement Range
From 2 pg/mL up to 200 pg/mL (typical)

Usage Type
Laboratory research and diagnostics

Function
Detection and quantification of human IL-6

Storage Instructions
Store at 2-8C

Accuracy
High, validated for human samples

Instruments Type
Immunoassay kit

Equipment Type
ELISA Kit

Material
High-quality microtiter plate and reagents

Condition
New

Technology
Enzyme-linked immunosorbent assay (ELISA)

Portable
Yes

Wall Mounted
No

Real-Time Operation
No

Operating Type
Manual

Use
Quantitative measurement of human Interleukin-6 (IL-6) in biological samples

Power Source
Not required (manual assay)

Dimension (L*W*H)
Standard ELISA kit box size

Weight
Approx. 1 kg

Color
White microplate; colorless to various colored reagents

Sample Type
Serum, plasma, or cell culture supernatant

Standard Curve Range
2200 pg/mL

Intended Use
For research use only, not for diagnostic procedures

Regulatory Status
CE marked for research use

Incubation Temperature
Room temperature (1825C)

Sensitivity
<2 pg/mL

Detection Method
Colorimetric (450 nm)

Assay Time
2-4 hours

Catalog Number
950.030.096

Calibration
Standard provided in kit

Total Tests Per Kit
96 determinations

About DIACLONE HUMAN IL-6 ELISA KIT

Diaclones range of high-quality immunoassay kits provide the sensitivity and performance required for the best efficient and sensitive detection of cytokines, chemokines, growth factors and their associated receptors, amongst others.

Diaclone ELISA are highly sensitive assays designed for in-vitro quantitative and qualitative determination of natural and recombinant proteins from serum, plasma and cell culture supernatant.

Control of our own mAb development coupled with robust manufacturing procedures ensures excellent performance and batch to batch consistency.

High Sensitivity (HS) kits for the detection of low expressing proteins with sensitivities below 1.5pg/ml.

With over 1000 immunoassay kits and corresponding kit components available, Diaclone can provide an ELISA solution to match your needs: for cytokine or CD marker studies, for Cytokines Release Syndrome monitoring (CRS) and many other applications.

The kits are available in multiple assay formats, sizes or species reactivity. For example, IL-6 detection can be performed by ELISA on human samples or murine or rat samples, with or without plates, or alternatively utilizing matched antibody pairs for ELISA or ELISpot.

High Sensitivity and Accuracy

This ELISA kit offers exceptional sensitivity (<2 pg/mL) and accuracy for detecting human IL-6, enabling researchers to reliably quantify low concentrations in biological samples. The kit is validated for human serum, plasma, and cell culture supernatant, and covers a wide dynamic range (2-200 pg/mL), ensuring applicable results across multiple research areas.

Ready-to-Use and Easy to Store

All reagents in the DIACLONE HUMAN IL-6 ELISA KIT are ready-to-use and supplied in a standard 96-well plate format. This design streamlines laboratory workflow and minimizes preparation time. The components should be stored at 2-8C for up to 12 months from the manufacturing date, preserving reagent integrity and performance.

Perfect for Laboratory Research

Intended for research use only, this ELISA kit is an excellent tool for scientists studying the role of IL-6 in health and disease. Its manual, colorimetric detection (450 nm) process does not require electrical power, making it portable and ideal for diverse laboratory settings in India.

FAQ's of DIACLONE HUMAN IL-6 ELISA KIT:

Q: How do I perform the assay using the DIACLONE HUMAN IL-6 ELISA KIT?

A: To conduct the assay, add your samples (serum, plasma, or cell culture supernatant), provided standards, and controls to the 96-well microtiter plate. Follow the kit's step-by-step protocol, incubating at room temperature and measuring color development at 450 nm after 2-4 hours. Detailed instructions are provided with the kit.

Q: What is the sensitivity and measurement range of this ELISA kit?

A: The DIACLONE HUMAN IL-6 ELISA KIT offers a sensitivity of less than 2 pg/mL and a standard curve range of 2-200 pg/mL, ensuring precise quantification even at very low analyte levels.

Q: When should I use this ELISA kit in my research?

A: Use this kit when you need to quantitatively measure IL-6 in human biological samples to study immune response, inflammation, or related research fields. It is designed for research purposes and not intended for diagnostic procedures.

Q: Where can the DIACLONE HUMAN IL-6 ELISA KIT be used?

A: This portable, manual kit is suitable for use in research laboratories, academic institutions, and biotech facilities across India. It does not require power or wall mounting, making it flexible for different research environments.

Q: What is the process for calibrating the assay?

A: Calibration is straightforward. The kit includes ready-to-use IL-6 standard solutions, which should be run alongside your samples to generate a standard curve for accurate quantification.

Q: How should the kit be stored to ensure optimal performance?

A: Store all contents of the kit at 2-8C as recommended. This ensures reagent stability and optimal assay performance for up to 12 months from the date of manufacture.

Q: What are the main benefits of using this ELISA kit for human IL-6 detection?

A: Key benefits include high sensitivity and accuracy, quick assay turnaround, validated detection in human samples, and convenient, ready-to-use reagents suitable for various research applications.