CALBIOTECH VITAMIN D ELISA TEST KIT

CALBIOTECH VITAMIN D ELISA TEST KIT Specification

Features
High sensitivity, Specific for 25(OH) Vitamin D, Ready-to-use reagents, User friendly protocol

Function
Vitamin D quantification in patient samples

Accuracy
High, correlates well with LC-MS/MS

Measurement Range
4-120 ng/mL (may vary by kit batch)

Usage Type
Diagnostic Laboratory Use

Storage Instructions
Store at 2-8C in a cool, dry place, away from light

Shelf Life
12 months

Instruments Type
Clinical Diagnostic Kit

Equipment Type
ELISA Test Kit

Material
High quality plastic and reagents

Condition
New

Technology
Enzyme-Linked Immunosorbent Assay (ELISA)

Portable
Yes

Wall Mounted
No

Real-Time Operation
No

Operating Type
Manual

Use
Quantitative determination of 25(OH) Vitamin D in human serum or plasma

Power Source
Not required for kit; ELISA reader requires electricity

Dimension (L*W*H)
Standard kit size (Boxed, dimensions may vary by kit size)

Weight
Approx. 0.5 to 1 kg depending on kit size

Color
White (kit box), Multiple (labels and solution colors)

Sample Type
Serum or Plasma

Assay Format
Competitive ELISA

Reagent Stability
Stable until expiration date when properly stored

Result Interpretation
Requires microplate reader at 450 nm

Sensitivity
Less than 1.5 ng/mL

Number of Tests per Kit
96 tests

Application
Clinical biochemistry, Endocrinology, Research use

Lot Number & Expiry Date
Printed on the kit label

Incubation Time
About 2 hours total

Control & Calibrators
Included in the kit

About CALBIOTECH VITAMIN D ELISA TEST KIT

Calbiotech Vitamin D ELISA Kit

The CALBIOTECH VITAMIN D ELISA TEST KIT is a quantitative enzyme-linked immunosorbent assay (ELISA) kit designed for the determination of total 25-hydroxy (25-OH) Vitamin D in human serum and plasma.

Key Characteristics:

Manufacturer: Calbiotech, Inc.
Type: ELISA test kit
Tests Kit: Vitamin D (25-OH)
Packaging Size: 96 Wells
Sample Volume: 10 l/well
Species: Human
Storage and Stability: Store at 2-8 C; for research use only, not for use in humans

Technical Specifications:

Standard Range: 2.5-150 ng/ml
Analytical Sensitivity: 2.5 ng/ml

Applications:

Research use only
Quantitative determination of total 25-OH Vitamin D in human serum and plasma
Suitable for hospital and diagnostic laboratory settings

Reliable Detection with High Sensitivity

The CALBIOTECH Vitamin D ELISA Test Kit provides robust detection of 25(OH) Vitamin D with sensitivity below 1.5 ng/mL. Designed for serum or plasma samples, it ensures accuracy comparable to LC-MS/MS. With a wide measurement range and stable reagents, the kit delivers dependable outcomes for diagnostic and research settings. The inclusion of controls and calibrators further supports result reliability.

User-Friendly and Efficient Protocol

This kit is engineered for convenience with ready-to-use reagents and a clear, manual protocol suitable for diagnostic laboratories. The total assay can be completed within about 2 hours, making it efficient for routine or urgent vitamin D testing. Portably packaged and requiring only standard laboratory microplate readers, it easily integrates into existing workflows.

Long Shelf Life and Optimal Stability

Each kit has a shelf life of 12 months when stored at 2-8C in a cool, dry place away from light. The stability and quality of reagents are maintained up to the expiration date as indicated on the lot label, ensuring confident usage throughout the kit's lifespan.

FAQ's of CALBIOTECH VITAMIN D ELISA TEST KIT:

Q: How is the CALBIOTECH Vitamin D ELISA Test Kit used in clinical and research settings?

A: The CALBIOTECH Vitamin D ELISA Test Kit is used to quantitatively determine 25(OH) Vitamin D levels in human serum or plasma, supporting both routine diagnostics and specialized research in clinical biochemistry and endocrinology.

Q: What types of samples are compatible with this kit?

A: This test kit is specifically designed for use with human serum or plasma samples, providing flexibility for various clinical or laboratory protocols.

Q: When should results be read and what equipment is required?

A: Results should be interpreted after completing the approximately 2-hour incubation process, using a microplate reader set at 450 nm for optimal accuracy.

Q: Where should the kit be stored to maintain reagent stability?

A: The kit should be stored at 2-8C in a cool, dry place and protected from light to preserve reagent quality and stability until the expiration date.

Q: What is the process for performing the Vitamin D assay with this kit?

A: The kit follows a competitive ELISA format: add samples, controls, and calibrators to the plate, incubate with ready-to-use reagents, wash, add substrate, and read absorbance at 450 nm using a microplate reader.

Q: What are the benefits of using this ELISA kit over alternative methods?

A: This kit offers high sensitivity and accuracy, user-friendly protocols, and results comparable to LC-MS/MS, making it suitable for reliable and efficient laboratory testing of vitamin D levels.